To facilitate mutagenesis study, it is necessary to be able to derive mutation targets and associated substitution rates in the sequence of interest regardless of the availability of corresponding structure. It is also important to obtain these data depending on the specific aims of the mutation process, e.g. modification towards more/less thermostable structure.

The mBlosum server determines candidate positions for mutations and derives position-specific substitution rates in any protein sequence. Different sets of complete genomes collected according to their phylogeny or specificity of environments and complete set of non-redundant sequences can be used in calculations depending on the experimental task. mBlosum will then use the input protein sequence to Blast against the selected set, create a multiple aligmment and use this to derive the position specific substitution rates.

Alternatively you can provide your own multiple sequence alignment that represents the specific aim of the mutation process.


Ma BG, Berezovsky IN. (2010) The MBLOSUM: a server for deriving mutation targets and position-specific substitution rates. Journal of Biomolecular Structure & Dynamics, ISSN 0739-1102 Volume 28, Issue Number 3, (2010) [ http ]